HPLC法测定骨筋丸胶囊中蛇床子素的含量

HPLC determination of osthole in Gujinwan Capsule

目的:使用HPLC法测定骨筋丸胶囊中蛇床子素的含量。方法:采用ODS-C18(150mm×4.6mm,5μm)流动相:甲醇-水(75∶25),流速1.0ml/min,检测波长322nm。结果:蛇床子素线性范围为12.5～200μg/ml,平均回收率为99.56%,精密度RSD为0.54%,重现性RSD为0.27%,稳定性RSD为0.48%。结论:本法用于测定蛇床子素的含量方法准确简便。

Objective：To determination the quantitative of osthole in Gujinwan Capsule by using HPLC. Methods：Column ODS-C18（150 mm×4.6 mm,5 μm）. Mobile phase,Methane-water（75：25）,flow rate at 1.0 ml/min,wavelength 322 nm. Results：The linear range was 12.5-200 μg/ml,the average recovery was 99.6%,precision RSD was 0.54%,repetition RSD was 0.27%,stability RSD was 0.48%。 Conclusion：This method is accurate and reliable.