摘要
目的探讨胆固醇酯转运蛋白(CETP)基因的一个新发多态位点——L296Q与散发型阿尔茨海默病(SAD)易感性的关系。方法应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)、变性高效液相色谱(DHPLC)和测序等方法检测107例SAD患者和115名健康老年人L296Q基因多态性。结果无论是哪种方法均未发现SAD组和对照组存在Q等位基因或LQ和QQ基因型。结论在研究人群中未发现此多态位点,可能与研究人群间的种族、年龄差异或技术问题有关。
Objective To investigate the association between CETP(L296Q) polymorphism and sporadic AD (SAD) in Northern Han-Chinese.Methods L296Q genotype was determined in 107 cases and 115 age and gender-matched controls by three methods such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP),denaturing high performance liquid chromatography (DHPLC) and DNA sequencing.Results None of the AD and healthy subjects was carriers for this variant.Conclusions This difference from a previous study may be due to the differences in age and race,or a technical problem.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2010年第15期2100-2102,共3页
Chinese Journal of Gerontology
基金
国家"973"重点基础研究发展规划资助项目(2006CB500701)
关键词
胆固醇酯转运蛋白
基因多态性
阿尔茨海默病
高密度脂蛋白
Cholesteryl ester transfer protein
Polymorphism
Alzheimer’s disease
High-density lipoprotein