马齿苋多糖的抗氧化活性研究

Antioxidant activities of polysaccharide from Portulaca Oleracea L.

目的研究马齿苋多糖(POP)的抗氧化作用。方法采用体外实验研究POP对超氧阴离子、羟自由基、DPPH的清除作用以及对H2O2诱导的红细胞氧化溶血和大鼠肝匀浆脂质过氧化的保护作用。结果 POP对超氧阴离子和羟自由基具有良好的清除作用,IC50分别为4.29和1.97μg/mL,对有机自由基DPPH的作用很弱。POP对自发性脂质过氧化和H2O2诱导的脂质过氧化具有良好的保护作用,但对H2O2诱导的红细胞氧化溶血作用较弱。结论 POP具有显著的体外抗氧化活性。

Purpose To study the antioxidant activities of polysaccharide from Qortulaca Oleracea L.（POP）.Methods By measuring the scaveoging activities of POP on superoxide radical,hydroxyl radical and DPPH.The protective effects of POP on H2O2 induced hemolysis of rat erythrocytes and liqid peroxidation of rat-liver homogenate were measured using an in vitro system.Results POP had strong scavenging effect on superoxide radical with an IC50 of 4.29 μg/mL,and on hydroxyl radical with an IC50 of 1.97 μg/mL,but had lower effect on DPPH.POP showed a significant protective effect on lipid peroxodation of rat-liver homogenate but have little inhabitation effect on hemolysis of rat erythrocytes induced by H2O2.Conclusion POP shows a significant antioxidant activity in experiments in vitro.