HPLC-ELSD法测定阿拉伯胶酶解液中L-阿拉伯糖含量

A HPLC-ELSD method for determinating the content of L-arabinose enzymolyzed from the arabic gum

目的建立用高效液相色谱-蒸发光散射检测仪(HPLC-ELSD)测定阿拉伯胶酶解液中L-阿拉伯糖含量的方法。方法采用HypersilNH2色谱柱(4.6mm×250nm,5μm),以乙腈-水(80:20)为流动相,流速1mL/min;蒸发光散射检测器检测参数为:漂移管温度100℃,氮气流速为2.5L/min。结果 L-阿拉伯糖与其他糖分的分离效果良好,L-阿拉伯糖的线性回归方程的相关系数为0.9993,线性范围为0.1025～1.025mg/mL,重复性实验RSD为2.03%,回收率为98.68%。结论该法简便,快速,准确,适用于阿拉伯胶酶解液中L-阿拉伯糖含量的测定。

Purpose To develop an efficient method to determine the content of L-Arabinose enzymolyzed from the arabic gum.Methods Different kinds of sugar could be separated on Hypersil NH2 column with the mobile phase of acetonitrile-water (80:20) at a flow rate of 1 mL/min.The conditions of evaporative light scattering detector were set as followings:the temperature of drift tube was 100 ℃ and nitrogen gas was used as carrier with the flow rate of 2.5 L/min.Results L-Arabinose could be separated from other saccharides.The correlation coefficient of linear regression equation for L-arabinose was 0.999 3.The reproducibility was RSD of 2.03%.The average recovery was 98.68%.Conclusion This method for determination of key saccharides enzymolyzed from arabic gum is simple,fast and accurate.