摘要
目的:探讨自研血细胞分析仪配套稀释液,研制适用于Sysmex XT-1800血细胞分析仪的稀释液,以替代昂贵的原装试剂.方法:对原装试剂进行理化分析,根据分析结果配制两者PH值,电导率,离子浓度等指标相近的稀释液,并做一系列对比试验.结果:自配和进口稀释液理化指标一致,检测85份抗凝静脉样本,所得11项主要指标均数相近,经配对资料t检验,p值均〉0.05,相关系数均〉0.90,而且自配试剂重复性、稳定性良好.结论:自配Sysmex XT-1800稀释液完全可以替代原装稀释液.
Objective: To study a new type of diluent reagent for Sysmex XT-1800 blood analyzer can be replaced the expensive original reagent. Methods:Using the physical and chemical methods to analyse elements of original dilunent reagent and comparing the conductivity rate, osmotic pressure,ion concentration, as and as a serious test of comparisons were made. Conclusion The physical-chemical index of imported and home-made diluents reagent were not found different, for eighty-five of anticoagulant venous blood sample determinated by Sysmex XT-1800 analyzer with some imported and heme-maded diluent reagents,the average of eleventh assay paraments weres similar in the t test and p valves greater than 0.05. The correlation coefficient valves were greater than 0.90. The home-made reagent have good repeatability and stability. Conclusion:The home-made diluent reagent of ? Sysmex XT-1800 analyzer can thoroughly replace the imported reagent.
出处
《按摩与康复医学》
2010年第24期12-13,共2页
Chinese Manipulation and Rehabilitation Medicine