儿童呼吸道合胞病毒的分子生物学检测

The child respiratory tract gathered the butcher virus the molecular biology to examine

目的： 建立呼吸道合胞病毒分子生物学检测方法,了解临床儿童呼吸道病毒感染常见病原体分布情况.方法：建立分子生物学方法,检测标本中的呼吸道合胞病毒,与免疫学检测方法（IFA）进行对照,并利用该方法进行该病毒在深圳市的流行性分析.结果：1012例标本中,呼吸道感染患儿中RSV感染达到17.9%（181/1012）,本方法与IFA检测方法具有较高的相关性和符合率,均可用于呼吸道合胞病毒的早期诊断.结论：本呼吸道合胞病毒分子生物学检测方法可用于儿童呼吸道合胞病毒感染的临床诊断.

Objective：Establishes the respiratory tract to gather the butcher virus molecular biology examination method, the understanding clinical child respiratory tract virus infection common pathogen distributed situation. Methods：The establishment molecular biology method, in the examina- tion specimen respiratory tract gathers the butcher virus,carries on the comparison with immunology examination method（IFA）,and carries on this virus using this method in Shenzhen＇s epidemic analysis. Results：In 1012 example specimens, the respiratory tract feeling catches the RSV infection to achieve 17.9^（181/1012）,this method and the IFA examination method have the high relevance and the coincidence rate, available gathers the butcher virus in the respiratory tract the early diagnosis. Conclusion： This respiratory tract gathers the butcher virus molecular biology examination method available to gather the butcher virus infection in the child respiratory tract the clinical diagnosis.