摘要
目的探讨二氮嗪对氧糖剥夺(OGD)PC12细胞凋亡的保护作用及其机制。方法体外培养PC12细胞株,以OGD、二氮嗪、5-羟葵酸(5-HD)处理,分为A组(正常对照组),B组(氧糖剥夺组),C组(氧糖剥夺+二氮嗪组),D组(氧糖剥夺+二氮嗪组+5-HD组),采用Annexin V-FITC/PI双染流式细胞分析仪检测凋亡率,应用免疫荧光染色和Western blot检测Bcl-2蛋白表达水平,观察二氮嗪对氧糖剥夺PC12细胞凋亡的保护作用。结果氧糖剥夺后B,C,D组PC12细胞凋亡细胞数增加,C组与B、D组比较差异均有显著性(P<0.01)。B,C,D组Bcl-2蛋白表达增加,C组达到高峰。C组与A、B、D组比较差异均有显著性(P<0.01)。结论二氮嗪能抑制氧糖剥夺PC12细胞凋亡,这一作用机制可能是增加Bcl-2表达来实现其保护作用的。
Aim To study the effect of diazoxide on the apoptosis of PC12 cells induced by oxygen-glucose deprivation（OGD）and expression of Bcl-2 protein.Methods Cultured PC12 cells,treated with OGD,diazoxide and 5-HD,were divided into A（control group）,B（OGD group）,C（OGD＋diazoxide group）and D（OGD＋diazoxide＋5-HD group）.Neuronal apoptosis was detected by Annexin V-FITC/PI double-dyed flow cytometry,and the expression of Bcl-2 protein was detected by immunofluorescence and Western blot.Results The number of apoptotic PC12 cells increased after OGD in B,C,D group.C group and B,D groups were significantly different（P〈0.01）.The expression of Bcl-2 protein in B,C and D group was higher than that of A group,and that in C group was significant higher than that of A,B and D group（P〈0.01）.Conclusion Diazoxide has a protective effect on the apoptosis of PC12 cells induced by OGD,which might be correlated with the up-regulated expression of Bcl-2 protein.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2010年第7期930-933,共4页
Chinese Pharmacological Bulletin
基金
辽宁省科学技术计划资助项目(No2008225022)
