脑力苏胶囊对血管性痴呆小鼠GSH-Px表达的影响

Mental Su Capsule on Vascular Dementia in Mice the Expression of GSH-Px

目的:研究脑力苏胶囊对血管性痴呆(VD)小鼠一般情况和GSH-Px表达的影响,探讨该方药对大脑的部份保护作用及其防治机制。方法:采用反复夹闭颈总动脉方法建立血管性痴呆小鼠模型。将造模成功后的小鼠随机分成5组:假手术组;模型对照组;西药治疗组;脑力苏胶囊高剂量组和脑力苏胶囊低剂量组。造模成功后,给药第11天观察小鼠一般情况变化,第14天后快速断头取脑,制成10%的组织匀浆液,4℃下离心,取血浆测定谷胱甘肽过氧化物酶(GHS-Px)活性的变化。结果:实验结果显示造模后的VD小鼠体重增长速度均有不同程度的降低,各治疗组小鼠的体重增加明显;治疗组GSH-PX水平与模型组比较均出现不同程度的升高,以脑力苏各组表现明显(P<0.01)。结论:脑力苏胶囊可能通过提高脑组织中GHS-Px活性,从而提高血管性痴呆小鼠的学习记忆能力。

Objective：To study the brain-Soviet capsule on vascular dementia （VD） mice were normal and GSH-Px expression,to explore some of the prescriptions on the brain protective effect and its control mechanisms. Methods：The method of repeated occlusion of common carotid artery to establish a mouse model of vascular dementia. After the success of the modeling of the mice were randomly divided into five groups：sham operation group; model control group; western medicine treatment group; mental Su capsule high dose group and mental Su capsule low dose group. Modeling after the success of the first 11 days of mice administered a general change in circumstances,the first 14 days after rapid decapitation,brain was removed tissue made of 10% of the liquid,4 ℃,under centrifugation,taking plasma glutathione peroxide HRP （GHS-Px） activity changes. Results：The results showed that after modeling of the VD growth rate of body weight of mice were reduced to varying degrees,all treated groups of mice gained weight significantly; treatment group,GSH-PX levels were compared with model group,there are different degrees of increased Su heads to the performance of each group significantly （P0.01）. Conclusion：The brain-Soviet capsule can enhance the brain tissue through the GHS-Px activity,thereby enhancing vascular dementia learning and memory in mice.