肠必清栓对UC模型大鼠肠黏膜内COX-2 NF-κB及TGF-β1的影响

Effects of Changbiqing Suppository on the Expression of COX-2 NF-κB and TGF-β1 in Colonic Mucosa of Rats with Ulcerative Colitis

目的:通过检测肠必清栓治疗后UC模型大鼠肠组织中COX-2、NF-κBp65、TGF-β1表达的变化,研究肠必清栓对UC的治疗机制。方法:用2,4-二硝基氯苯免疫加醋酸局部灌肠法建立的UC模型大鼠48只随机分为5组:A模型对照组(8只)、B肠必清栓低剂量组(10只)、C肠必清栓中剂量组(10只)、D肠必清栓高剂量组(10只)、E柳氮磺吡啶药物对照组(10只)另设F正常对照组(6只)。给药治疗14天后处死,留取结肠组织检测组织中细胞因子COX-2、NF-κBp65、TGF-β1的表达。结果:模型组大鼠肠组织中COX-2、NF-κBp65和TGF-β1的表达明显增高(P<0.01);与模型组比较,用肠必清栓治疗后,给药各组大鼠的COX-2、NF-κBp65和TGF-β1表达显著降低(P<0.01),肠必清栓低剂量组与中、高剂量组的COX-2、NF-κBp65和TGF-β1表达差异显著(P<0.01),SASP组的COX-2、NF-κBp65评分与肠必清栓低剂量组间差异无显著性意义,而TGF-β1评分与肠必清栓中、高剂量组相当无统计学差异。结论:肠必清栓可有效下调结肠组织中TGF-β1的表达并通过抑制NF-κBp65的活性而下调COX-2的表达,这可能为其治疗UC的作用机制。

Objective：Observing the effect of Changbiqing suppository on the expression of COX-2 NF-κBp65and TGF-β1 in colonic mucosa of rats with ulcerative colitis to reveal the possible mechanismfor treatment.Methods：Fifty SD UC model rats induced by dinitrochlorobenzene （DNCB）and acetic acid were randomly assigned into five groups from A to E：UC model group（A）,low moderate large dose interventiongroup（B C D）,SASP group（E）,another six rats as normal control group（F）besides. The fresh colons of the rats were got after 14 days of treatment and the antigen expression of COX-2 NF-κBp65 and TGF-β1 were evaluated. Results：In modle rats with UC,the antigen expression of COX-2 NF-κBp65and TGF-β1 were significantly higher（P〈0.01） when compared with the control group.Compared with the UC group,the expression of COX-2 NF-κBp65and TGF-β1 in group B to E decreased markedly（P〈0.01）andin a dose-dependent mannerin group B to D. In contrast,no significant differences in expression of COX-2and NF-κBp65 were observed in group B and Eas well as TGF-β1in group C D and E. Conclusions：Changbiqing suppository may play its role by down-regulating the expression of cytokine such as COX-2 NF-κBp65 and TGF-β1.