嗅三针对血管性痴呆大鼠学习记忆功能及海马ET和CGRP含量的影响

Effect of Xiusanzhen on learning-memory abilities,contents of hippocampus ET and CGRP in vascular dementia rats

目的探讨嗅三针对血管性痴呆(VD)大鼠学习记忆功能及海马ET和CGRP含量的影响。方法成年SD雄性大鼠随机分为正常对照组、VD模型组、VD嗅球损毁模型组和嗅三针组,每组10只。四血管阻断法制作大鼠VD模型,手术电凝法制作大鼠嗅球损毁模型,采用嗅三针电刺激方法对VD嗅球损毁模型组和嗅三针组进行治疗,42d后测试大鼠水迷宫学习记忆能力并测定海马ET和CGRP含量。结果与嗅三针组比较,VD模型组和VD嗅球损毁模型组大鼠平均逃避潜伏期和平均游泳路程均延长,差异有统计学意义(P<0.01);与VD模型组和VD嗅球损毁模型组比较,嗅三针组大鼠海马ET含量降低,CGRP含量升高,差异均有统计学意义(P<0.05);以上各项指标,VD模型组与VD嗅球损毁模型组比较,差异均无统计学意义(P>0.05)。结论嗅三针能增强血管性痴呆大鼠学习记忆功能、并且能够降低海马ET的含量同时提高海马CGRP的含量,其治疗效应的发挥与嗅觉传导通路密切相关。

Objective To explore the effect of Xiusanzhen,a method of acupuncture,on learning-memory abilities,and contents of hippocampus endothelin（ET） and calcitonin gene-related peptide（CGRP） in vascular dementia（VD） rats.Methods The SD rats were randomly divided into control,VD model,VD ＋olfactory bulb lesion model and Xiusanzhen groups,10 rats pre group.VD models were established by 4-vessel occlusion,olfactory bulb lesion models by electric coagulation.Xiusanzhen was used to treat VD＋olfactory bulb lesion models and Xiusanzhen groups,and after 42 days,morris maze learning and memory abilities were tested and the contents of hippocampus ET and CGRP were detected.Results Compared with control group,the average escape latency and swim distance prolonged significantly in VD model group and VD ＋olfactory bulb lesion model group（P0.01）;compared with VD model group and VD ＋olfactory bulb lesion model group,the content of hippocampus ET in Xiusanzhen group decreased（P0.05）,and the content of hippocampus CGRP increased（P0.05）;but the differences of all the indexes above between VD model and VD ＋olfactory bulb lesion model group were not significant（P0.05）.Conclusion Xiusanzhen therapy can strengthen the learning -memory abilities,decrease hippocampus ET content and increase hippocampus CGRP content in VD rats,which may be closely related to the olfactory pathway.