HPLC测定市售青黛中靛蓝和靛玉红的含量

Determination of Indigo and Indirubin in Indigo Naturalis by HPLC

目的:建立青黛中靛蓝和靛玉红含量的测定方法。方法:采用HPLC定量分析,色谱柱为Diamonsil C18(150mm×4.6mm,5μm),靛蓝流动相为甲醇-水(60∶40),流速为1.0mL·min-1,检测波长为610nm;靛玉红流动相为甲醇-水(70∶30),流速为1.0mL·min-1,检测波长为292nm。结果:靛蓝在0.0296～0.296μg(r=0.9999)与峰面积呈良好线性关系,靛玉红在0.0106～0.1272μg(r=0.9999)与峰面积呈良好线性关系。结论:HPLC操作简便、结果可靠、重现性好、专属性强,可用于青黛中有效成分含量的测定。

Objective：To determine the contents of indigo and indirubin in indigo naturalis. Methods： The contents of indigo and indirubin were determined by HPLC. The chromatographic column was Diamonsil C18 （ 150mm × 4. 6mm,5μm）. The mobile phase was methanol-water（60：40） and the flow rate was 1.0mL·min^-1 with detection wavelength of 610nm for indigo. The mobile phase was methanol-water（70：30） and the flow rate was 1.0mL·min^-1 with detection wavelength of 292nm for indirubin. Results： The linear correlation of the indigo was good in the range of 0. 029 6 - 0. 296μg（ r = 0. 999 9）. The Linear correlation of the indirubin was good in the range of 0. 010 6 - 0. 127 2μg（r =0. 999 9）. Conclusion： HPLC method is simple and the result is reliable with good repeatability and can be used for the determination of the components in indigo naturalis.