食管鳞癌发生过程中TESTIN的表达变化及临床意义

Detection of testin in human esphageal squamous cell cancer tissues

目的研究食管鳞癌发生过程中TESTIN的表达变化及临床意义。方法应用免疫组织化学S-P法检测65例人食管鳞状细胞癌组织,40例癌旁正常食管黏膜组织,及40例不典型增生组织中TESTIN表达的差异,并分析TESTIN的表达与患者临床病理特征的关系。结果免疫组化结果显示TESTIN在32.3%(21/65)的食管鳞癌组织中呈阳性,不典型增生组织中70%(28/40)呈阳性,而在癌旁正常食管黏膜组织中有87.5%(35/40)呈阳性(P<0.01);统计结果分析发现,TESTIN的表达与食管鳞癌分化程度有关,而与性别、年龄、肿瘤大小、TNM分期、淋巴结转移关系无统计学意义。结论 TESTIN基因的低表达在食管鳞癌的发生、发展过程中起一定的作用,可能是一种潜在的抑癌基因,并且可能成为食管鳞癌诊断和判断其恶性程度的一个指标。

Objective To investigate the expression of testin in human esphageal squamous cell cancer tissues,and to discuss its value in the development of esphageal squamous cell cancer（ESCC）.Methods The expression of testin in 65 matched-pairs of ESCC tissues and 40 atypical hyperplasia tissues were detected by immunohistochemistry methods.The correlations of testin to clinicopathologic features of ESCC were analyzed.Results The positive rate of testin protein was significant lower in ESCC than in atypical hyperplasia tissues and normal tissues（32.3% vs.70%.vs.87.5%,p0.01）.Testin expression was positive correlated with the differentiation of ESCC.Meanwhile,differences in gender,age,tumor size,TNM stage,lymph node metastasis and depth of invasion were not significant.Conclusion The decreased expression of testin gene may play an importmant role in the development of esphageal squamous cell cancer.And thus testin gene might be a novel candidate of tumor-suppressor.It may be an objective marker for prognostic factor and malignant level for ESCC.