肾脏损伤早期肿瘤坏死因子-α表达的实验研究

Experimental research of the expression of TNF-α in renal early trauma

目的探讨外源性肾上腺髓质素（ADM）对肾脏机械性损伤早期肿瘤坏死因子-α（TNF-α）表达的影响。方法选健康成年普通级Wistar大鼠104只,随机分为4组：对照组8只、创伤组32只、伤前给药组32只、伤后给药组32只,后3组的动物分别于创伤后1、6、12、24h各处死8只。采用自由落体打击仪直接打击大鼠脊肋区制作肾脏机械性损伤模型。ADM分别于创伤前或创伤后10min采用腹部注射法给药。实验动物采用快速心脏采血法处死。迅速解剖动物提取下肾脏标本,10%甲醛固定,免疫组化染色观察TNF-α在组织中的表达规律。结果创伤组于创伤早期TNF-α表达均高于对照组,外源性ADM早期（1、6h）可抑制TNF-α表达（P〈0.05）。结论 TNF-α在创伤早期即参与了肾组织炎症的急性期反应并加重损伤。ADM能降低巨噬细胞的白细胞趋化物的分泌水平,降低TNF-α的分泌。

Objective To investigate the effects of TNF-αexpression about mechanical renal trauma with extragenous ADM.Methods There were 104 healthy adult plain grade Wister rtas,randomly divided into four groups：8 in the group of control,32 in the group of trauma,32 in the group injected ADM before trauma,32in the group injected ADM post trauma.8 rats in the last three groups were excuted at 1,6,12,24 hpost trauma respectively.The experimental models of rat kidney with mechanical trauma were prepared by striking the area of rat skin reflecting by kidney with free dropping ferreous hammer.ADM administrated by intrapertoneal injection at 10 minutes before trauma or post trauma respectively.All rats were executed by drawing-out all the blood in their hearts.Renal tissue was investigated to study positive expression of TNF-α after SABC stained.Results The TNF-α expression of trauma group was more positive than that of control group in the wound early time.The extragenous ADM inhibited the expression of TNF-α（1,6h）（P〈0.05）.Conclusion The TNF-α participates in the kidney tissue acute inflammatory stage and aggravates the damage in early time.ADM can reduce the secretion level of leukocyte chemotaxi of macrophage and the expression of TNF-α.