枸杞多糖对诱导树突状细胞成熟和增强T细胞增殖的影响

Effect of inducing dendritic cell mature and stimulating T cell proliferation by lycium bararum polysaccharides

目的观察枸杞多糖对诱导树突状细胞（DC）成熟和T细胞增殖的作用。方法通过密度梯度离心法分离人外周血单核细胞,采用细胞因子体外培养7d后分别加入枸杞多糖,观察细胞形态变化,用免疫细胞化学法检测DC分子的表达,通过混合淋巴细胞反应检测细胞提呈抗原的能力。结果经枸杞多糖诱导的DC分子表达有明显变化（MHCⅡ＋、CD86＋、CD83＋）,差异有统计学意义（P〈0.05）,而经RPMI1640培养的DC分子表达与培养前无明显变化。经枸杞多糖诱导的DC能促进T细胞增殖能力（P〈0.05）。结论枸杞多糖能促进DC成熟和增强T细胞增殖能力。

Objective To investiage the effect of inducing dendritic cell mature and stimulating T cell proliferation by lycium bararum polysaccharides.Methods Monocytes obtained form human peripheral blood by density guadient centrifugation had been cultured in the presence of cytokines in vitro for seven days,and then was cultured in the presence of lycium bararum polysaccharides.Morphologic changes and molecule express of DC were observed by immunocytochemical method.The ability of antigenic presentation was examined by mixed lymphology reaction.Results DC molecule express had marked change by lycium bararum polysaccharides：MHCⅡ^＋,CD86^＋,CD83^＋（P〈0.01）,DC molecule express has not marked change by RPMI 1640.DC induced by lycium bararum polysaccharides could reinforce effective T cells proliferation（P〈0.01）.Conclusion Lycium bararum polysaccharides can promote the maturity of DC and reinforce proliferation of T cells.