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脂质体电化学发光免疫传感器用于人IgG的检测 被引量：1

Detection of Human IgG Based on Liposomes Electrochemiluminescence Immunosensor
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摘要 实验构建了一种检测人免疫球蛋白G(人IgG)的竞争型免疫传感器.采用层层组装的方法将IgG抗体固定在金纳米粒子修饰的金电极表面,基于待测IgG与IgG抗原标记的、内部包裹Ru(bpy)23+的脂质体之间的竞争,实现对人IgG的检测.结果表明,电化学发光强度与人IgG的浓度在0.1-3 ng/mL范围内呈良好的线性关系,线性回归方程为y=803.1-206.2x(ng/mL)(n=6,R=0.99),检出限为0.05 ng/mL.对人血清中IgG检测,结果令人满意. A competitive electrochemiluminescence（ECL） immunosensor was developed by immobilizing goat-anti-human immunoglobulin G（hIgG） antibody on a gold nanoparticles（gold NPs） modified Au electrode.The assay is based on the competition between the antigen and antigen-tagged liposomes.Under the optimal conditions,the ECL intensity exhibited a linear response to hIgG in the range from 0.1 to 3 ng/mL（n=6,R=0.99） with a detection limit of 0.01 pg/mL（S/N=3）.
作者 李玲 王海燕 孙东艳 龚武 王伦
机构地区 安徽师范大学化学与材料科学学院安徽省生物传感省级重点实验室
出处 《安徽师范大学学报（自然科学版）》 CAS 北大核心 2010年第3期258-261,共4页 Journal of Anhui Normal University(Natural Science)
基金 国家自然科学基金(20705002)
关键词 电化学发光 人免疫球蛋白G 免疫传感器 脂质体 electrochemiluminesence human immunoglobulin G immunosensor liposome
分类号 O657.1 [理学—分析化学]
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参考文献9

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共引文献6

同被引文献7

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安徽师范大学学报（自然科学版）
2010年 第3期

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