银杏叶提取物对肝肺综合征大鼠ET-1和CGRP的影响

Study on Effects of Ginkgo Biloba Extract on ET-1 and CGRP in Hepatopulmonary Syndrome in Rats

目的:探讨银杏叶提取物(GBE)对肝肺综合征(HPS)大鼠血管扩张因子内皮素-1(ET-1)和降钙素基因相关肽(CGRP)的影响。方法:雄性成熟SD大鼠32只,随机平均分为4组:模型组、正常对照组、GBE低剂量组、GBE高剂量组,采用胆总管结扎术(CBDL)成功制备大鼠HPS模型,喂养3周后统一处死,用放射免疫分析法检测大鼠血浆和肝组织、肺组织匀浆中ET-l和CGRP的水平。结果:在大鼠HPS形成过程中,模型组、GBE低剂量组、GBE高剂量组血浆(血清)和肝、肺组织匀浆中ET-1、CGRP水平显著升高,经GBE治疗后显著下降,且GBE高剂量组更为显著。结论:GBE可降低HPS大鼠血浆(血清)和肝、肺组织匀浆中ET-1和CGRP水平,且高剂量比低剂量的治疗效果明显。

Objective：To explore the effects of ginkgo biloba extract on endothelin-1 and calcitonin gene-related peptide in HPS rats.Methods：Thirty two male SD rats were randomly and averagely divided into 4 groups：model group;nomal control group;GBE low-dosage group;GBE high-dosage group.HPS model was induced by CBDL.They were all sacrificed after 3 weeks.The concentrations of ET-1 and CGRP in plasma,liver and lung tissues were detected by radioimmunoas-say（RIA）.Results：During the pathogenesis of HPS,the levels of ET-1 and CGRP in plasma or serum,1iver and lung tissues in model group,GBE low-dosage group and GBE high-dosage group were increased and strikingly reduced after treated by GBE,especially in GBE high-dosage group.Conclusion：GBE can reduced the levels of ET-1 and CGRP in plasma or serum,1iver and lung tissues in HPS rats,and it＇s effect of treatment in GBE high-dosage was more obvious than that in GBE low-dosage.