模拟失重状态下大鼠心血管中枢组织基因组变化研究

Research on the Gene Changes of Rat Cardiovascular Central Tissue under Simulated Weightlessness

目的拟通过动物模拟失重模型,研究模拟失重条件下心血管调节功能改变发生的分子机制。方法利用基因芯片技术,检测5例模拟失重大鼠和5例对照大鼠心血管中枢组织基因芯片的变化情况,从基因层面上揭示模拟失重心血管功能变化的实质。结果基因芯片结果显示:模拟失重状态导致心血管中枢部位ADP核糖基化因子样2(ADP-ribosylation factor-like2,Arl2)、孕酮和adipoQ受体家族成员Ⅳ(progestin and adipoQ receptor family member Ⅳ,paqr4)、丙酮酸脱氢酶激酶同工酶1(pyruvate dehydrogenasekinase,isozyme1,pdk1);促生长素抑制蛋白(somatostatin,sst)、跨膜蛋白酶(transmembraneprotease、tmprss5)、丝氨酸5(脊突蛋白)serine5(spinesin)发生了改变。结论失重状态下,心血管功能的变化可能与Arl2、Paqr4、Pdk1、Sst、Tmprss5涉及的转导通路的改变有关。

Objective To study the molecular mechanism of cardiovascular regulatory functional changes in rats under simulated weightlessness condition. Methods The gene changes of cardiovascular central tissue in five control rats and the five models were examined with gene chip technology to display the essence of cardiovascular functional changes. Results The gene chips showed that ADP-ribosylation factor-like2,progestin and adipoQ receptor family member IV,pyruvate dehydrogenasekinase,isozyme1;somatostatin,sst, transmembraneprotease, tmprss5 and serine5（spinesin）were changed in the cardiovascular center under simulated weightlessness. Conclusion Under weightlessness the changes of cardiovascular function are related to the signal channels about Arl2, Paqr4, Pdk1, Sst and Tmprss.