多坝式空间细胞培养室设计参数的微重力实验验证

Validation of Design Parameters of the Multi-bar Space Cell Culture Chamber under Microgravity Experiments

目的利用失重飞行提供的微重力环境验证多坝式空间细胞培养室的设计参数。方法设计了三坝式和五坝式两种结构的插片细胞培养室,在失重飞机抛物线飞行中,通过研究培养室内流场形态、液流更换效率及流速对室内细胞的影响,对其在微重力下的适用性进行了验证。结果三坝或五坝式结构均能在室内隔离形成有效液流通路,确保微重力环境下培养室内溶液的有效更替;插片式设计在方便取出细胞样品用于后续分析的同时,可将细胞培养片固定在培养室底部防止刮蹭细胞;微重力下50μL/min和150μL/min两种换液流速均不会损伤细胞。结论综合考虑空间细胞培养的高容积利用率及短操作时间等需求,插片式三坝细胞培养室结构及150μL/min的换液流速更符合应用要求。

Objective To validate the design parameters of the multi-bar space cell culture chamber under microgravity environment. Methods Space cell culture chambers of two configurations,three-bar and five-bar were developed and tested aboard the parabolic flight aircraft. Chambers with inserted slide and three-bar or five-bar were validated by investigating the flow visualization, liquid replacement efficiency and cell morphology in them during the flight. Results Both three and five bars in the cell culture chambers could form efficient liquid channels to provide nutrient or reagent, and no difference of the liquid replacement efficiency was observed among all groups during flight. The fixed slots in the chamber were available for inserting and anchoring the slide seeded with cells on the chamber base, which was convenient for taking cell samples out for following analysis.The cell culture slide attached to the chamber base stably during whole parabolic flight and no cell scratch was induced by the floating of the slide.Neither 150 μL/min nor 50 μL/min of the liquid replacement velocity would damage the cells in the chambers. Conclusion With consideration of the requirements for high cubage-utilization-ratio and low time-cost of cell culture in orbit, the chamber with three-bar and inserted slide is approved to be more reasonable and the liquid flow velocity of 150 μL/min is more appropriate.