高压氧对减压病小鼠脑组织IL-1β和IL-10含量的影响

Effects of Hyperbaric Oxygen on the Contents of IL-1β and IL-10 in the Brain Tissue of Mice with Decompression Sickness

目的探讨高压氧对减压病小鼠脑组织IL-1β和IL-10含量的影响。方法小鼠随机分为对照组、减压病组和高压氧组。减压病组和高压氧组小鼠经600kPa压缩空气暴露后,用1min快速减压至常压。高压氧组小鼠在快速减压1h后接受高压氧处理。用酶联免疫吸附法检测减压病组和高压氧组小鼠在快速减压6h后以及对照组小鼠脑组织IL-1β和IL-10含量。结果减压病组小鼠脑组织IL-1β含量显著高于对照组(P<0.01);高压氧组小鼠脑组织IL-1β含量显著低于减压病组(P<0.05)。对照组、减压病组和高压氧组之间小鼠脑组织IL-10含量无显著性差异(均P>0.05)。结论减压病早期小鼠脑组织存在促炎细胞因子和抗炎细胞因子之间的失衡,高压氧可有效降低促炎细胞因子水平,可能有助于减轻快速减压后继发性脑损伤。

Objective To investigate the effects of hyperbaric oxygen （HBO）on the contents of IL-1β and IL-10 in the brain tissue of mice with decompression sickness. Methods Mice were randomly divided into control group, decompression sickness group and HBO group. Mice in decompression sickness group and HBO group were exposed to 600 kPa compressed air for 60 min, and then they were rapidly decompressed to normal pressure in 1 min. Mice in HBO group were treated by HBO 1 h after rapid decompression. The contents of IL-1β and IL-10 were measured with enzyme-linked immuno-sorbent assay in the brain tissue of mice in decompression sickness group and hyperbaric oxygen group 6 h after rapid decompression as well as control group. Results The content of IL-1β in the brain tissue of mice in decompression sickness group was higher than that in control group（P0.01）. The content of IL-1β in the brain tissue of mice in HBO group was lower than that in decompression sickness group （P0.05）.There was no significant change in the content of IL-10 in the brain tissue of mice among control group, decompression sickness group and hyperbaric oxygen group. Conclusion There is unbalance between pro-inflammatory and anti-inflammatory cytokines in the brain tissue of mice during early period after rapid decompression. HBO may reduce the content of pro-inflammatory cytokine effectively, which may conduce to relieve the secondary brain injury of mice after rapid decompression.