HPLC法测定筋痛消酊中大黄素和大黄酚的含量

Determination of Emodin and Chrysophanol in Jintongxiao Tincture by HPLC

目的采用HPLC法测定筋痛消酊中大黄素和大黄酚的含量。方法色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),流动相为甲醇-0.1%磷酸溶液(80∶20),流速为1.0 ml/min检测波长为288 nm。结果大黄素、大黄酚分别在4.28～24.78μg/ml,3.65～21.25μg/ml(r=0.999 8,0.999 9)范围内有良好的线性关系,平均回收率分别为100.12%和100.04%(n=7),RSD分别为2.19%与1.98%。结论本法简便、快速、准确,可有效地控制药品质量。

Objective To establish an HPLC method for the determination of emodin and chrysophanol in Jintongxiao tincture.Methods Diamonsil C18（4.6 mm×250 mm,5 μm） column was used.Methanol and 0.1% phosphoric acid（80∶20） was used as the mobile phase,with flow rate 1.0 ml/min and the detection wavelength 288 nm.Results The linear range were 4.28～24.78 μg/ml,3.65～21.25 μg/ml（r=0.999 8,0.999 9）.The average recovery rates were 100.12% and 100.04%（n=7）,the RSD were 2.19% and 1.98%,respectively.Conclusion The method is convenient,sensitive,and accurate.It can be used for the quality control of Jintongxiao tincture.