石菖蒲不同部位对戊四唑点燃大鼠药效学研究

Pharmacodynamics Study of Different Parts of Acori tatarinowii for Pentylenetetrazol Kindling Rats

目的:研究石菖蒲不同部位对戊四唑点燃癫痫模型大鼠的作用。方法:SD大鼠80只,ip戊四唑溶液35 mg·kg-1体重,隔天1次,共14次。点燃成功的大鼠,分8组,每天ig 1次,分别给予石菖蒲挥发油50 mg·kg-1、石菖蒲去油水提液高、中、低剂量分别为28,14,7 g·kg-1;β-细辛醚100 mg.kg-1,α-细辛醚70 mg·kg-1,阳性对照组给予丙戊酸钠126 mg·kg-1治疗,模型组给予同量生理盐水。另设正常组5只,正常喂养,不作任何处理。治疗36 d后用同剂量戊四唑点燃测试药效,记录惊厥发作的潜伏时间、发作的级别和次数、湿狗样次数。结果:石菖蒲去油水提液高剂量组治疗后3级发作次数较治疗前减少(P<0.05),α-细辛醚治疗后2级发作次数较治疗前减少(P<0.05),3级发作次数较治疗前减少(P<0.01)。结论:石菖蒲水溶性部分和α-细辛醚有减少戊四唑点燃大鼠癫痫发作次数的作用。

Objective：To study the antiepileptic effect of different parts of Acori tatarinowii for pentylenetetrazol kindling rats.Method： Eighty SD rats were intraperitoneally injected with pentylenetetrazol solution at a dosage of 35 mg·kg-1 once every the other day for 14 times.The successfully kindled rats for epilepsy were then divided into 8 groups and drugs were ig given once a day accordingly.Volatile oil from Acorus tatarinowii was given at 50 mg·kg-1;the high doses of liquid extract with the volatile oil removed from A.tatarinowii at 28 g·kg-1;the medi-um dose of liquid extract with the volatile oil removed at 14 g·kg-1;the low-dose at 7 g.kg-1;β-asarone at 100 mg.kg-1;α-asarone at 70 mg·kg-1.Positive control group was given sodium valproate at 126 mg.kg-1.The normal group of 5 rats was given a normal ration without any special treatment.The model group was given the same volume of physiological saline.36 days after the above mentioned treatments,the rats were injected intraperitoneally with the same dose of pentylenetetrazol,a test was done to determine the efficacy： the potential seizure observation time,the level and frequency of attacks,and the number of wet dogs.Result：The frequency of third-level seizure attack in the high dose group of A.tatarinowii water extracts without oil was reduced significantly（P 0.05）.The fre-quency of the second-level attack after the treatment by means of α-asarone was reduced significantly（P 0.05）,and the frequency of the third-level attack was also reduced significantly（P 0.01）.Conclusion：The ingredients for antiepileptic effect in A.tatarinowii are its water-soluble parts and α-asarone.