毛樱桃乙醇提取物的试验研究

Study on Ethanol Extracts of Prunus tomentosa

目的:通过现代科学方法确定毛樱桃抗冻伤乙醇提取的最佳工艺。方法:采用正交实验设计法优选毛樱桃的最佳提取工艺,采用兔耳冻伤模型进行提取物抗冻伤实验研究。结果:毛樱桃加8倍量95%乙醇,采用索氏提取器回流提取2次,每次1.5h,此条件下的提取率最高,该提取物在兔耳冻伤试验中能明显减轻兔耳局部冻伤组织红肿等炎症反应,防止水泡产生和组织坏死,对兔耳轻、中度冻伤有显著的治疗作用。结论:本研究确定了毛樱桃最佳醇乙提取工艺,且提取物具有显著的抗炎及抗冻伤作用。

Objective：To determine the best extraction process of effective part of Prunus tomentosa Thunb by modern scientific methods. Method：Orthogonal design was used to screen and optimize the extraction conditions of P. tomentosa,and animal model of frostbite were used to observe the effect of the extractions. Result：The best extraction condition was：8 times 95% ethanol was added and extracted 2 times （1. 5 h /time） by Rope extractor. The extraction could significantly reduce the inflammation of swelling and prevent blisters and tissue necrosis at the rabbit＇s ears. The extract had the significant therapeutic effect for the mild to moderate frostbite of rabbit＇s ear. Conclusion：The optimized extraction method was established and the extract had moderate effect of antiinflammation and anti-frostbite. The results also provide the scientific data for further studying on the new traditional medicine.