高效液相色谱法测定新疆紫草毛状根中乙酰紫草素的含量

HPLC Determination of Acetylshikonin in Hairy Roots of Arnebia euchroma

目的:建立高效液相色谱法(HPLC)测定新疆紫草毛状根中乙酰紫草素含量的方法。方法:采用YMC ODS-A C18色谱柱(4.6mm×150mm,5μm),流动相为乙腈-水-甲酸(700∶300∶0.5);检测波长275nm;流速1.0mL·min-1;柱温35℃。结果:乙酰紫草素对照品在51.55～1031.0mg·L-1与峰面积线性关系良好,相关系数r=0.9999,平均回收率为99.81%,RSD2.00%(n=9)。结论:高效液相色谱法操作简单快速,重复性好,可用于新疆紫草毛状根中乙酰紫草素含量的测定。

Objective：To establish a method for the determination of acetylshikonin in the Hairy Roots of Arnebia euchroma （ Royle） Johnst. Method：The RP-HPLC system consisting of YMC-Pack ODS-A C18 column （4. 6 mm × 150 mm,5 μm） and a mixture of acetonitrile solution and water solution and formic acid solution（700∶ 300∶ 0. 5）as the mobile phase was adopted; the detection wavelength was 275 nm; the flow rate was set at 1. 0 mL ·min^-1and the column temperature was at 35 ℃. Result：The linear range of acetylshikonin was 51.55-1 031.0 mg·L^ -1（r = 0. 9 999）. The average recovery （n = 9） was 99. 81%. Conclusion：The method is quick,simple and reproducible for the determination of acetylshikonin in Hairy roots of A. euchroma.