HPLC测定益心康泰胶囊中大黄素大黄酚含量

Determination of Emodin and Chrysophanol in Yixin Kangtai Capsule by HPLC

目的:建立高效液相色谱法测定益心康泰胶囊中大黄素、大黄酚含量。方法:以Hypersil-ODS2C18(4.6mm×250mm,5μm)为分析柱,流动相为甲醇-0.1%磷酸(80∶20),检测波长为254nm,流速为1mL·min-1,柱温20℃,进样量10μL。结果:大黄素在0.04～0.80mg·L-1线性关系良好(r=1.0000),平均回收率为99.5%(RSD1.81%);大黄酚在0.1102～2.204mg·L-1线性关系良好(r=1.0000),平均回收率为99.1%(RSD1.80%)。结论:样品处理方法简便,灵敏度高,结果准确。

Objective：To establish a HPLC method for the determination of emodin and chrysophanol in yixinkangtai capsules. Method：Hypersil-ODS2 C18（4. 6 mm × 250 mm,5 μm） column was used with methanol-0. 1% phosphoric acid （80∶ 20） as mobile phase; the detection wavelength was at 254 nm,flow rate was 1 mL· min -1,column temperature 20 ℃ ,injection volume was 10 μL. Result：The linear range of emodin and chrysophanol was 0. 04-0. 80 mg· L^ -1 （ r = 1. 000 0） and 0. 110 2-2. 204 mg · L^ -1 （ r = 1. 000 0） respectively. The average recovery was 99. 5% with RSD 1. 81% and 99. 1% with RSD 1. 80% respectively. Conclusion：The sample preparation method is simple,sensitive and accurate.