新疆紫草羟基萘醌类化学成分的研究及对PDE_4的抑制作用

Hydroxynaphthoquinone Compounds From Arnebia euchroma and Their Inhibitory Activities of Phosphodiesterase 4

目的:研究新疆紫草羟基萘醌的主要成分及对PDE4酶的抑制作用。方法:用硅胶柱层析、制备HPLC和制备TLC等方法分离纯化新疆紫草中羟基萘醌化合物,通过理化性质和光谱数据进行结构鉴定。HPLC方法测定主要羟基萘醌类化合物对PDE4酶的抑制活性。结果:分离鉴定了7个化合物,分别为去氧阿卡宁(1)、阿卡宁(2)、乙酰阿卡宁(3)、β,β′-二甲基丙烯酰阿卡宁(4)、α-甲基丁酰阿卡宁(5)、异丁酰阿卡宁(6)和β-乙酰氧基异戊酰阿卡宁(7)。结论:化合物2,5,6为首次从新疆紫草中分离得到。首次评价了新疆紫草羟基萘醌类成分对PDE4酶的抑制作用。

Objective：To investigate the Hydroxynaphthoquinone of Arnebia euchroma（Royle） Johnst and their inhibitory activities on phosphodiesterase 4. Method：Hydroxynaphthoquinones were isolated and purified by chromatographic methods. Their chemical structures were elucidated on the basis of physicochemical properties and spectral data and their inhibitory activity on phosphodiesterase 4 were detected by HPLC. Result：7 Hydroxynaphthoquinones were isolated and identified as：deoxyalkannin 1,alkannin 2,acetylalkannin 3,β,β-dimethylacrylalkannin 4,α-methybutyrylalkannin 5,isobutyrylalkannin 6,β-acetoxyisovalerylalkannin 7. Conclusion：Compounds 2,5,6 were isolated fromArnebia euchromafirst time. Their inhibitory activities on phosphodiester -ase 4 were evaluated by HPLC method for first time.