人肾小管上皮细胞HK-2的培养及生长曲线测定

Culturing of Human Tubular Epithelial Cells and Measuring Cell Growth Curve

目的:通过细胞计数法绘制人肾小管上皮细胞株HK-2细胞的生长曲线,了解HK-2细胞的生长特性,为相关实验研究提供参考。方法:将HK-2细胞用含10%胎牛血清的DMEM/F12(1∶1)培养基培养,每隔24h在倒置显微镜下观察细胞生长范围,并进行计数,连续观察7d后,以存活细胞数(万/mL)对培养时间(h或d)作图,即得生长曲线。结果:细胞呈立方形铺路石样贴壁生长,细胞间连接紧密。细胞数目在培养第2d开始增加,但在第1～3d增加缓慢,第3～6d细胞数目急剧增加,并进入指数生长期,在第6d时细胞达到饱和密度(100%),在第7d时细胞数目显著下降。结论:在1～3d细胞生长处在潜伏期,3～6d细胞进入大量分裂的指数生长期,第6d达到饱和后,细胞生长则进入衰退期。

Objective：To investigate growth feature of human tubular epithelial cells（HK-2） through cell growth curve. Method：The HK-2 cells were cultured by DMEM / F12（1∶ 1） with 10 % fetal bovine serum. The cells morphology was observed through the microscope,and cells were counted per 24 hours. After observing 7 days the cell growth curve was drawed based on day and amount. Result：We found that HK-2 cells crawling like stone road on the bottle surface. The cell amount began increased on the second day,but the speed was slower during the first day to the third day. The amount increased rapidly from the third day to the sixth day,and the cells were in the exponential phase of growth. On the sixth day,the cells were in saturation density（100% ）. However,the amount decreased rapidly on the seventh day. Conclusion：The cells were in the incubation period from the first day to the third day,and in the exponential phase of growth from the third day to the sixth day,and in the decline phase on the seventh day.