摘要
从腐烂水果、果园土壤、堆肥、生物肥料中分离出120株菌株,旨在从中筛选出高产聚半乳糖醛酸酶的菌株。采用染色透明圈法初筛获得20株水解圈直径(Dp)与菌落直径(Dc)比值较大的菌株。再通过液体发酵,测定粗酶液酶活力复筛出5株高产聚半乳糖醛酸酶菌株,其酶活力分别达到17.89、17.68、18.37、14.04、13.56U/mL。最后经过菌落形态、显微形态和生理生化特征的鉴定,有3株菌株属于同一菌种,结果为:C1、C2、C3-土曲霉(Aspergillus terreus),G1-互隔交链孢霉(Alternaria alternate),H4-黑曲霉(Aspergillus niger)。
One hundred and twenty strains,isolated from rotten fruits,soil,rice straw compost and biologic fertilizer,were screened for polygalacuronase production.All the strains were positive for pectinase activity in a translucent circle assay,as evidenced by clear translucent halos.Twenty strains presented considerable polygalacturonase activity.The cultivation of the best five strains（C1,C2,C3,G1,H4） by liquid-state fermentation,resulted in high activities of polygalacturonase enzyme.The enzyme activity were respectively 1 7.89,17.68,18.37,14.04,13.56 U/mL.Then they were classified respectively as Aspergillus terreus（C1,C2,C3）,Alternaria alternate（G1）,Aspergillus niger（H4） by the analysis of their colony morphology,micrology morphology,and physiology characteristic.
出处
《食品科技》
CAS
北大核心
2010年第8期7-12,17,共7页
Food Science and Technology
关键词
聚半乳糖醛酸酶
筛选
鉴定
酶活力
polygalacturonase
screening
identification
enzyme activity
