槟榔中槟榔碱的超声波提取工艺研究

Study on ultrasonic extraction process of arecoline from betel nut

运用超声波辅助提取法从槟榔中提取槟榔碱。以槟榔碱的提取量为考察指标,用高效液相色谱法测定其含量,在单因素实验的基础上,采用L9(34)正交试验得出槟榔中槟榔碱的最佳提取工艺为:乙醇浓度85%,料液比为1∶8,提取温度65℃,碱化pH值8。槟榔碱得率为0.1987%,提取物的槟榔碱纯度为98.1%(W)。

To use ultrasonic-assisted extraction process to extract arecoline from betel nut.The content of the arecoline which was used as evaluating criteria,was determined by HPLC.Based on the results of single factor experiments,the optimum extracting process of arecoline from betel nut was optimized by L9（34） orthogonal test.The optimized extraction process was as follows：the concentration of ethanol was 85%;the ratio of solid to liquid was 1：8;the extraction temperature was 65 ℃;the pH value of alkalization was 8.The extraction rate of arecoline was 0.1987%.The purity of arecoline extraction was 98.1%（W）.