黄瓜香提取物对实验性肝纤维化大鼠肝内Ⅰ、Ⅲ型胶原及生长转化因子β_1表达的影响

Effect of VDG on the expression of collagen alphalⅠ、Ⅲ and TGF-β_1 in rats with hepatic fibrosis

目的:探讨黄瓜香对肝纤维化大鼠肝组织Ⅰ、Ⅲ型胶原及生长转化因子β1(TGF-β1)表达的影响。方法:将48只大鼠随机分为正常对照组、模型组、生理盐水组和黄瓜香干预组,全自动生化分析仪检测ALT、AST、ALP,HE染色显示肝的显微结构,SABC法免疫组织学染色显示Ⅰ、Ⅲ型胶原及TGF-β1。结果:与模型组和生理盐水组比较,黄瓜香组ALT、AST、ALP含量均明显降低(P<0.01);黄瓜香组肝小叶结构较正常,少数见纤细的纤维间隔,有少量炎性细胞浸润;模型组、正常对照和黄瓜香干预组Ⅰ、Ⅲ型胶原及TGF-β1表达的平均灰度值比较均有显著性差异(P<0.01)。结论:黄瓜香提取物可明显减少纤维化组织面积,其在肝纤维化治疗方面有一定的开发价值。

Objective：To study the effects of VDG on the expression of collagen alphal Ⅰ、Ⅲ and transforming growth factor-β1（TGF-β1） in rats with hepatic fibrosis.Methods：Forty-eight ratswas randomLy divided into four groups：normal control group,model control group,normal sodium group and interrupt group.The contents of ALT,AST and ALP was measured by full automatic biochemical analyzer,hepatic microstructure was showed by HE staining,the expression of collagen alphal Ⅰ,Ⅲ and TGF-β1 was showed by immunohistochemistry dyeing.Results：Compared with the model group,the contents of ALT,AST and ALP remarkably decreased（P0.01）.In interrupt group,the hepatic lobe structure was normal,the slender textile fiber gap was minority,and there was the few inflammatory cell infiltration.There was significant difference in the average grey degree of collagen alphal Ⅰ,Ⅲ and TGF-β1 among normal control group,model control group and interrupt group（P0.01）.Conclusion：The alcohol extract from VDG showed the protective effects for liver fibrosis and could alleviate the degree of degeneration and necrosis induced by CCl4.