细胞色素C与辅酶Q10联产工艺研究

Technical study on co-producing Cytochrome-C and Coenzyme Q_(10)

细胞色素C和是辅酶Q10促生物氧化酶类生化药物。实验从猪心中联合提取细胞色素C和辅酶Q10。猪心绞碎后以酸性水搅拌粗提,过滤分离为上清液和猪心渣,上清液经人造沸石吸附,洗脱,盐析,三氯乙酸沉淀,除盐精制,细胞色素C得率134.08mg/kg猪心。猪心渣用正己烷萃取,丙酮冷析,经矽酸镁层析,无水乙醇结晶,辅酶Q10得率为90.23mg/kg猪心。

Cytochrome C and Coenzyme Q10 are enhancing bio-oxidases of Biochemical Drugs.This experiment extracted Cytochrome C and Coenzyme Q10 from pig heart.After grinded,the pig heart was extracted by sour water.Then filtered two parts：the supernatant and the residue.After the supernatant was adsorbed by permutite,eluted,Salted-Out,deposited with TAC,desalinated and purified,we get the Cyt C yield is 134.08 mg/kg.The pig heart residue was extracted with n-hexane,separated with cold acetone,laminar analyzed with magnesium trisilicate,dissolved by anhydrous alcohol,we get the Coenzyme-Q10 yield is 90.23 mg/kg.