玉米抗氧化肽的制备及纯化

Preparation and Purification of Antioxidant Peptide from Corn Gluten Meal

对玉米蛋白粉Alcalase酶解工艺进行了优化,优化得到的工艺条件为:pH值8.0,温度65℃,时间2h,酶浓度4 000U/g,底物浓度2%,在此工艺条件下,玉米蛋白粉水解度为73.25%。玉米蛋白水解液经AmberliteCG-120阳离子交换层析分离和Sephadex G-25凝胶层析分离后,收集DPPH清除能力最高的组分经RP-HPLC二次分离后获得了一种纯度较高的抗氧化肽组分,采用HPLC-MS分析其分子质量为1 147.2 u。

Enzymatic hydrolyzing technology of corn gluten meal was investigated. The results showed that the optimum conditions are： pH 8.0, temperature 65℃ , enzyme concentration 4 000 U/g, substrate concentration 2% , and hydrolyzing time 2 h. Under the optimizing technology parameters, the degree of hydrolysis was 73.25%. The hydrolysate were separated by Amberlite CG-120 and Sephadex G-25. Most of DPPH savenging activity profiles were deserved and separated by RP-HPLC twice, and a 1147.2 Da peptide was deserved with higher purity.