Infusa Sleeve导管血管内局部给药的实验研究

Experimental Study on Intravascular Local Drug Delivery With Infusa Sleeve Catheter

目的：评价ＩｎｆｕｓａＳｌｅｅｖｅ（ＩＳ）导管局部给药的可行性。方法：１３只家兔随机分成Ａ组（ｎ＝８）和Ｂ组（ｎ＝５），给药压力分别为６０７．９ｋＰａ（６个标准大气压）（Ａ组）和２０２．６ｋＰａ（２个标准大气压）（Ｂ组），用标准血管成形导管损伤腹主动脉下端后，经ＩＳ导管向腹主动脉下端球囊损伤处局部注入１ｍｌ放射性同位素锝标记的甲氧基异丁基异腈（９９ｍＴｃ－ＭＩＢＩ）注射液。术后３０分钟、６小时、２４小时分别处死动物后，取两组动物靶点血管（腹主动脉下端）、心、肝、骨骼肌、小肠、血液、非靶点血管（肺动脉）组织，用γ计数仪计数后称组织重量。结果：Ａ组和Ｂ组给药后３０分钟靶点血管局部的放射强度分别占给药总量的４９．２５０％和２２．０００％、分别是血液的４５倍和８倍、是非靶点血管的７０倍和１２倍。靶点血管局部放射强度Ａ组大于Ｂ组（Ｐ＜０．０５）。６小时和２４小时后靶点血管局部放射强度占给药总量的０．３４３％（Ａ组）和０．２５５％（Ａ组）。结论：ＩＳ导管可经血管局部靶向给药。ＩＳ导管局部导入的药物浓度呈压力依赖性。

Objective:To assess the feasibility of the Infusa Sleeve (IS) catheter for the delivery of an agent into arterial wall at the site of percutaneous transluminal coronary angioplasty (PTCA) injury. Methods:Thirteen rabbits were randomly divided into two groups (A and B).Following a lesion dilatation of the wall of abdominal aorta by a standard angioplastic catheter,1 ml of Technetium[ 99m Tc] methoxy isobutyl isonitrile injection ( 99m Tc MIBI) was locally delivered to the injured site with IS catheter,at 607 9 kPa(6 atm) (group A, n =8) and 202 6 kPa (2 atm) (group B, n =5) pressures,respectively.The rabbits were sacrificed at 30 minutes,6 hours and 24 hours after the drug delivery.The target artery,control artery (pulmonary artery),cardiac muscle,liver,intestine and skeletal muscle were surgically isolated and scintillation counting and weighing were performed. Results:Thirty minutes following 99m Tc MIBI delivery,the radioactivity(Te cpm)of the target vessel wall as a percentage of in vitro value was 49 250% in group A,and 22 000% in group B;It was about 45 fold and 8 fold higher than that of the blood and 70 fold and 12 fold higher than that of control artery in group A and group B,respectively.The difference between the two groups was signficant ( p <0 05).The other 2 time point results showed that the radioactivity remained detectable (0 255%) up to 24 hours. Conclusions:It is possible to deliver agent to the local vessel wall via IS catheter;Agent delivery via the IS is pressure dependent.Evidence of 99m Tc MIBI delivery persists at least 24 hours.