旁路活化补体降低宿主抗感染条件与机制的实验研究

Condition and mechanism of host antiinfect capacity induced by bypass activated complement:experimental study

目的为证明旁路活化补体降低吞噬细胞功能，了解宿主抗感染能力下降的条件与机制。方法分离人中性粒细胞（ＰＭＮ）和大鼠枯否细胞（ＫＣ）；ＰＭＮ和ＫＣ培养单层加上酵母多糖活化人血清（ＺＡＨＳ）；观测吞噬细胞胞内杀菌力（ＩＣＢＡ）、超氧阴离子（Ｏ－２）、酸性磷酸酶（ＡＣＰ）指标动态水平；以抗人Ｃ３Ｃ５血清（ＡＨＣ３Ｃ５Ｓ）中和阻断ＺＡＨＳ进行反证。结果００７～００９ｍｌＺＡＨＳ处理组，ＫＣ胞内Ｏ－２水平在３小时前高于对照组值，然后逐渐下降。００３～００５ｍｌＺＡＨＳ６小时略有下降。ＩＣＢＡ其动态变化与Ｏ－２的相似，但００３～００５ｍｌ４小时后略有下降。ＡＣＰ水平只降无升，随ＺＡＨＳ用量增加，下降幅度越低，以上各指标至６小时降至最低。０１１ｍｌＺＡＨＳ处理组４小时后约８５％～９０％ＫＣ破裂脱落。ＺＡＨＳ作用ＰＭＮ的指标动态基本类似ＫＣ的动态，只是ＰＭＮ指标在２小时之前升高，然后逐渐下降，以００５～００９ｍｌＺＡＨＳ对ＰＭＮ指标影响最大。阻断组Ｏ－２、ＡＣＰ、ＩＣＢＡ在６小时测定值显著高于ＺＡＨＳ处理组，但仍低于正常对照。结论ＺＡＨＳ使吞噬细胞功能下降与Ｃ３Ｃ５碎片作用有关，在大中剂量作用时间较长的条件?

Objective To verify host antiinfect capacity induced by bypass activated complement. Methods A monolayer culture of human PMNs and rats kupffer cells (KCs) were added with zymosan activated human serum (ZAHS) and 3 parameters were measured. ZAHS had been neutralized in tube with antiserum against human C 3 and C 5. Results In 0.07 0.09 ml ZAHS and 1 3h, intracellular bactericidal activity (ICBA) showed a peak but significant top value at 2nd hour. The value dropped markedly with time and significantly in 4 6 h. Superoxide ions (O - 2) dynamics was similar to ICBA. Acid phosphatase (ACP) curve distinguished from the former′s with monotonous decrease (1 6 h) and more deep. With 0.11 ml ZAHS after 4 h, about 80% of KCs were fallen off. Three parameters of PMNs were decreased and reached the lowest point in the 6 th hour after the adding 0.05～ 0.09 ml of ZAHS. After blocking test with antihuman C 3 and C 5 serum, the values of ICBA, O - 2 and ACP surpassed those in the experimental group. Conclusion The possible machanism of the origination of the harmful effects of ZAHS is suggested. Before KCs and PMNs undertake phagocytosis, they release the bactericidal and inflamagenic (O - 2 and ACP) so the intracellular bactericidal activity of KCs and PMNs are decreased. The accumulation of KCs and PMNs in tissues can demage themselves and the adjacent tissues barrier, which will lead to the occurrence of infection.