摘要
目的研究信号调节蛋白SIRPα与肝细胞肝癌的发生及侵袭之间的联系。方法应用Northern杂交及免疫组织化学技术检测了42对肝细胞肝癌和相应癌旁肝组织内SIRPα基因表达的变化,并结合各组织标本的病理学特点进行分析。结果SIRPα的3.9kb转录子及2.5kb转录子分别在21例、27例肝癌组织内表达水平明显下降。直径>5cm的大肝癌SIRPα的表达下降率显著高于直径≤5cm的小肝癌,有癌栓形成的肝癌SIRPα的表达下降率显著高于无癌栓的肝癌。SIRPα的表达下降与患者年龄、肿瘤分级、包膜完整性、血清AFP水平、是否有乙肝感染无明显相关性。免疫组织化学显示在正常肝细胞、胆管上皮细胞、Ito细胞、再生肝细胞、新生胆管细胞及部分肝细胞肝癌(HCC)细胞均有表达。表达下降的HCC细胞表现为阳性颗粒变淡、变少,部分HCC细胞或区域完全无染色。结论SIRPα尤其是2.5kb转录子在肝癌内表达下降,与肝癌的生长、侵袭密切相关,可能为肝癌发生发展过程中一个重要的负调控因子。
Objective To study the roles of signal regulatory protein α (SIRPα) in the development of
hepatocellular carcinoma (HCC). Methods 42 pairs of HCC and the surrounding noncancerous
hepatic tissues were used . The expression of SIRPα in various tissues was determined by
Northern blot analysis and immunohistochemistry assay, by which together with the pathologic
characteristics of each sample, the role of SIRPα was investigated. Results The expression of
SIRPα significantly decreased in 21 cases of samples (50.0%) to the 3.9kb transcript and 27
cases of samples (64.3%) to the 2.5kb transcript respectively. The SIRPα decreasing rate in
large HCC(>5cm in diameter)was higher than in small HCC (≤5cm in diameter) , and in HCC
with cancer embolus was higher than that of without cancer embolus . No association was found
between the SIRPα expression and the patient's age, tumor grade, tumor capsule, serum AFP
level, and HBV infection. Immunohistochemistry showed that all the normal hepatocytes,
cholangioepithelial cells, Ito cells, regenerated hepatocytes, new generated cholangioepithelial
cells and part of HCC cells were positive. The HCC tissues in which SIRPα was down
regulated showed positive particles becoming weaker and less, and some tumor cells and area
were completely negative. Conclusion The expression of SIRPα especially 2.5kb transcript is
down regulated in HCC, which is closely related with the tumor growth and invasiveness. SIRP
α may be an important negative regulator of HCC.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第4期268-270,共3页
National Medical Journal of China
基金
国家自然科学基金
关键词
肝细胞
癌
信号转递
基因表达
肝肿瘤
Hepatocellular carcinoma
Signal transduction Expression