活血解毒方含药血清对银屑病角质形成细胞增殖及细胞周期的影响

Effect of Huoxue Jiedu Recipe Medicated Serum on Proliferation and Cell Cycle Status of Keratinocytes of Psoriasis

目的观察活血解毒方对体外培养的银屑病角质形成细胞增殖及细胞周期的影响。方法根据血清药理学方法 ,用清洁级SD大鼠制备大、中、小剂量活血解毒方含药血清、地塞米松含药血清及空白血清,分别加入角质形成细胞培养体系中。培养48h后,用MTT法观察COLO-16细胞增殖情况;用流式细胞仪检测细胞周期分布。结果不同浓度活血解毒方明显抑制COLO-16角质形成细胞株的增殖,同时可改变细胞分裂周期各时相分布比例。结论中药活血解毒方通过抑制角质形成细胞的异常增殖对银屑病有治疗作用。

Objective To observe the effect of Huoxue Jiedu Recipe （Herbal recipe for activating blood and removing toxins） on proliferation and cell cycle status of keratinocytes cultured in vitro in psoriasis. Methods Using the serum pharmacologic technique, the clean SD rats were adopted to make the Huoxue Jiedu Recipe medicated serum （high, moderate, low dose）, Dexamethasone medicated serum, and blank serum, which were added into the keratinocytes culture system respectively. After incubated for 48 hours, the cell proliferation of keratinocytes was measured by MTT assay, and the cell cycle distribution estimated with flow cytometer （FCM）. Results The different density of Huoxue Jiedu Recipe could all inhibit significantly the generation of COLO-16 keratinocyte line and change the distribution proportion of each phase in the cell division cycle. Conclusion Huoxue Jiedu Recipe treats psoriasis through inhibiting the proliferation of keratinocytes.