HPLC测定脑组织和脑脊液中4种氨基酸类神经递质含量的方法改良研究

Modification of HPLC Assay for 4 Amino Acid Neurotransmitter Content in Brain Tissue and Cerebrospinal Fluid

谷氨酸（Ｇｌｕ）和γ－氨基丁酸（ＧＡＢＡ）等神经递质的代谢异常是诱发多种神经系统疾病的因素之一。由于神经递质在脑内的含量很低，改良建立了一种用ＨＰＬＣ荧光检测Ｇｌｕ、ＧＡＢＡ等４种神经递质的方法，以高丝氨酸为内标，流动相为磷酸缓冲液和甲醇，梯度洗脱２０ｍｉｎ，荧光检测。分离效果及线性关系良好，Ｇｌｕ、ＧＡＢＡ的相关系数分别为０．９９６９、０．９９９６；精密度ＣＶ批内３．４％，批间５．６％；其最小检测限分别为３．３ｐｍｏｌ、１．

Abnormal metabolism of neurotransmitters such as glutamate(Glu) and γ aminobutyric acid(GABA) might contribute to one of factors to induce the multiple central nervous system (CNS) disorders. An modified fluorometric method was established for the determination of 4 neurotransmitters such as Glu and GABA by HPLC assay. Homoserine served as internal standard,phosphate buffer solution and methanol as mobile phases, and gradient elution lasted 20 min with fluorescence detection. Separation and linear relationship were fine. Coefficients of correlation for Glu and GABA were 0.996 9 and 0.999 6, respectively. The precision was 3.4 % for interassay CV , and 5.6 % for intraassay CV . The limits of detection for Glu and GABA were 3.3 pmol and 1.6 pmol, respectively.