Bcl-xl和p53在人卵巢癌SKOV3细胞中表达及中药干预作用

The gene expression of Bcl-xl and p53 in human ovary cancer SKOV3 cells and interference of Chinese medicine

目的探讨Bcl-xl、p53基因在白毛藤诱导人卵巢癌SKOV3细胞凋亡中的作用。方法不同浓度白毛藤作用于细胞,荧光显微镜观察人卵巢癌SKOV3细胞凋亡作用,RT-PCR法检测Bcl-xl、p53基因表达量的变化。结果白毛藤能诱导人卵巢癌细胞凋亡,并且上调p53和降低Bcl-xl表达。结论白毛藤促进凋亡,其机制可能与激活p53、抑制Bcl-xl表达有关。

Objective：To investigate the effect of Bcl-xl and p53 gene on Human ovary cancer SKOV3 cells.Methods：Human ovary cancer cells were treated with various of Solamum lyratum Thunb,We observed cell apoptosis of SKOV3 cells by fluorescence microscope.We detected the levels of Bcl-xl and p53 gene expression in all groups by RT-PCR.Results：Solamum lyratum Thunb has obviously apoptosis-inducing effect s on Human ovary cancer SKOV3 cells and could effectively decrease the level of Bcl-xl gene expression and recrease the level of Bcl-xl gene expression.Conclusion：Solamum lyratum Thunb could induce apoptosis of SKOV3 cells probably through down regulating Bcl-xl gene expression and up regulating p53 gene expression.