保达新对缺血再灌注损伤大鼠心肌的影响

The study of effect of Prostavasin on myocardial ischemia-reperfusion injury rats

目的 观察保达新对缺血再灌注损伤心肌的保护作用.方法 将24只左冠状动脉前降支（LAD）结扎大鼠分成3组（假手术组、对照组、治疗组）,分别在心肌缺血30 min后再灌注60min,复灌前5 min静脉注射0. 9%生理盐水0.25 ml/kg;或保达新0 .5 μg/kg,检测血液中乳酸脱氢酶（LDH）、血浆丙二醛（MDA）、白细胞介素（IL）-6及IL-8的差异和各组间bcl-2、bax蛋白表达的差异.结果 对照组与其他两组比较LDH、MDA、IL-6及IL-8明显增加（P＜0.05）,治疗组中LDH、MDA、IL-6及IL-8较空白组明显增加（P＜0.05）.对照组与假手术组比较bcl-2、bax蛋白表达明显增加（P＜0.05）,治疗组中bcl-2表达较其他两组明显增加（P＜0.05）,bax表达较对照组明显减少（P＜0.05）.结论 保达新可通过减少脂质过氧化物产生、减轻过氧化反应,抑制细胞凋亡来保护缺血再灌注损伤的心肌.

Objective To investigate the protective effect of Prostavasin on Myocardial ischemia-reperfusion injury. Methods 24 left anterior descending coronary artery （LAD） ligated rats were divided into 3 groups （sham operation group,control group,treatment group）. Reperfused 60 min respectively after myocardial ischemia 30 min,5 min before reperfusion intravenous 0.9% saline 0.25 ml/kg or Prostavasin 0.5μxg/kg. Detected the differences between blood Iactate dehydrogenase （LDH）, plasma malondialdehyde （MDA） ,interleukin （IL）-6 and IL-8,and the differences of bcl-2,bax protein expression between each group. Results Compared with other two groups, LDH, MDA, IL-6 and IL-8 increased significantly in the control group （P〈0.05） ;LDH,MDA,IL-6 and IL-8 in treatment group more significantly increased than the blank group（P〈0.05）. Compared with the sham operation group protein expression of bcl-2 and bax were significantly increased in control group （P 〈 0.05）; in treatment group, the expression of bcl-2 significantly increased compared with the other two groups, bax expression were markedly reduced （P 〈 0.05）. Conclusion Prostavasin can protect the ischemic-reperfusion injury myocardium by reducing lipid peroxides and reducing peroxidatic reaction, inducing apoptosis.