基于光纤的双光子激光扫描荧光微内窥镜的新进展

Progress of Two-Photon Laser Scanning Fluorescence Microendoscope Based on Optical Fiber

由于高选择性、高穿透性和高分辨率,双光子显微术已经成为一种很好的非侵入式检测方法。基于光纤的双光子微内窥镜在对清醒行为动物的脑成像并达到细胞水平分辨率,对内部器官和活体动物光学实时成像的应用研究中具极大潜力。对建立微型双光子荧光内窥镜的技术方法进行了综述,对基于单模、多模、光子晶体光纤等方法传输激发光和荧光信号进行了分析,着重介绍了不同扫描方式和系统设计,并对检测方法的研究进展和应用前景进行了探讨。

Because of its high resolution,inherent optical sectioning and deep optical penetration,two-photon microscopy has been regarded as an excellent method for noninvasive detection.A potential clinical application of these microscopes based on optical fibers is their endoscopic use for optical biopsy of inner organs or brain of awake,behaving animals.The technology to build miniaturized fluorescence microendoscopes was reviewed.The different fibers used in two-photon microendoscope to propagate the excited laser and collect the fluorescence signal such as single mode,multi-mode and photonic crystal fibers were contrasted.Various scanning mode and system design were introduced.The research development and potential application were also discussed.