亚甲蓝光化学法灭活血液中HBV的初步探讨

An experimental study on the effect of methylene blue photochemistry to inactivate hepatitis B virus in blood

目的:研究亚甲蓝光化学法对HBV的灭活效果。方法:选择慢性乙型病毒性肝炎患者30例,抽取其静脉血并使用亚甲蓝光化学法灭活HBV,以500μmol/L亚甲蓝灭活病毒1.5h后评价灭活效果,并检测灭活前后红细胞功能。结果:患者血液中HBV DNA拷贝数由灭活前的(1.19×107±1.23×106)copies/ml减少至灭活后的(3.46×105±5.01×104)copies/ml(P<0.05);红细胞丙二醛由灭活前的(2.5±0.2)mmol/L增加至灭活后的(9.1±1.7)mmol/L(P<0.05),而2,3-二磷酸甘油酸、Na+K+-ATP酶、Ca2+-ATP酶、Mg2+-ATP酶、血浆游离血红蛋白等无明显变化。结论:亚甲蓝光化学法能降低血液中HBV载量,对红细胞功能无明显影响。

Objective： To investigate the effect of methylene blue photochemistry （MBP） to inactivate hepatitis B virus （HBV） in blood. Methods： Thirty patients with chronic hepatitis B were randomly enrolled into the study and blood samples were obtained. The blood was treated with methylene blue 80 μmol/L for 1.5 h, then inactivation effect of MBP to HBV in venous blood was observed, as well as the erythrocyte function before and after the inactivation treatment was performed. Results： After the inactivation treatment, HBV DNA copies decreased significantly while malondialdehyde increased markedly, with no significant changes in 2, 3-DPG, Na ^＋ K^ ＋ -ATPase, Ca^2＋ -ATPase, Mg^2＋ -ATPase and free hemoglobin. Conclusion： MBP can effectively inactivate HBV without affecting erythrocyte function.