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大鼠冠状动脉平滑肌细胞急性分离以及在离子通道研究中的应用 被引量:4

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摘要 目的:建立一种大鼠冠状动脉平滑肌的急性酶分离方法,用于血管平滑肌离子通道特性的研究。方法:采用木瓜蛋白酶,胶原酶分离大鼠冠状动脉平滑肌,用膜片钳全细胞记录通道电流。结果:本分离方法耗时少,酶量使用少,所获细胞数量多,状态正常,容易记录到正常的钾通道电流。结论:此方法为使用膜片钳记录血管平滑肌细胞的离子通道提供了较好的材料。
出处 《实用医学杂志》 CAS 北大核心 2010年第16期2916-2918,共3页 The Journal of Practical Medicine
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参考文献5

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