健脾泻浊方对亚急性镉染毒大鼠肾毒性影响的实验研究

An experimental study on the effect of Jianpi-xiezhuo prescription against the kidney subacute toxicity of cadmium in rats

目的探讨中药健脾泻浊方对亚急性镉染毒大鼠肾毒性的影响。方法无特定病原体级SD雄性大鼠37只,随机分为空白对照组(7只)和模型组(30只)。空白对照组予腹腔注射生理氯化钠溶液,模型组大鼠以腹腔注射氯化镉混合液的方法制备亚急性镉中毒模型。于造模成功后,模型组随后被分为氯化镉染毒对照组(10只)、染毒后低剂量治疗组(10只)、高剂量治疗组(10只)共3组。对3组大鼠分别灌胃给予生理氯化钠溶液(10ml.kg-1.d-1)和低剂量(4.23g.kg-1.d-1)、高剂量(8.46g.kg-1.d-1)的中药健脾泻浊方。各组于实验终末抽取血液并留取肝、肾组织测定镉水平,并测定血液中尿素氮(BUN)和肌酐(Cr)水平。结果氯化镉染毒对照组的血、肝、肾镉水平均高于空白对照组(P<0.01),血BUN也高于空白对照组(P<0.05)。采用中药健脾泻浊方治疗后,高剂量治疗组和低剂量治疗组的血镉、肝镉、肾镉与氯化镉染毒对照组比较差异均无统计学意义(P>0.05)。氯化镉染毒对照组、染毒后低剂量治疗组和高剂量治疗组各组组内的肝镉、肾镉分别与血镉比较差异均有统计学意义(P<0.01),肝镉、肾镉均较血镉高;氯化镉染毒对照组的肝镉、肾镉比较差异无统计学意义(P>0.05),但染毒后低剂量治疗组和高剂量治疗组组内的肝镉、肾镉比较差异均有统计学意义(P<0.01),肝镉较肾镉高。结论健脾泻浊方对亚急性镉染毒大鼠没有增加肾的毒性作用,其对体内镉的驱排作用也不明显。

Objective To investigate the effect of Jianpi-xiezhuo prescription against the kidney subacute toxicity of cadmium in rats.Methods 37 SD male rats were randomly divided into 2 groups：a control group （7 rats） and a model group （30 rats）.The controls were treated with NS ip for 5 days.The model group were treated with cadmium chloride solution（CdCl2＋ mercaptoethanol）ip for 5 days to produce subacute cadmium poisoning modeled rats.The model group were then divided into 3 groups：Cd-control group （10 rats）,low dose （LD,10 rats） and high dose （HD,10 rats） group.Cd-control group were treated with NS（10 ml·kg-1·d-1）,while LD and HD group were treated with Jianpi-xiezhuo prescription （4.23,8.46 g·kg-1·d-1）,ig.After the treatment,the contents of cadmium in blood,liver,kidney,and the contents of BUN and Cr in the blood were determined.Results Cadmium in blood,liver and kidney and the level of BUN in Cd-control group were higher than those in the control group（P0.01,P0.05）.After the treatment with Jianpi-xiezhuo prescription.,there were no significant difference of cadmium in blood,liver and kidney between Cd-control group and LD or HD treatment groups （P0.05）.However,there were significant difference of cadmium level in liver,kidney and in blood among 3 groups（P0.01）.The contents of cadmium in liver and kidney were higher than those in blood.There was no significant difference between cadmium in liver and in kidney in Cd-control group（P0.05）,but there was significant difference of cadmium in liver and in kidney between LD and HD groups（P0.01）.The content of cadmium in liver was higher than that in kidney.Conclusion Statistic evidence suggests that Jianpi-xiezhuo prescription does not increase kidney’s toxicity,and that there is no obvious effect on the elimination of accumulated cadmium in exposed rats,although there is some effect on cadmium redistribution in rats.