HPLC法测定无敌丹胶囊中龙血素A的含量

Determination of Loureirin A in Wudidan Capsules by HPLC

目的:建立用液相色谱法测定无敌丹胶囊中龙血素A的含量。方法:采用Agilent ZORBAX XDBC18柱(4.6×250mm 5μm),流动相为冰醋酸溶液(1→90)—乙腈(63∶37),流速1.0mL.min-1,检测波长260nm。结果:线性范围6.58～65.8ug.m L-1,r=0.99999;平均回收率为99.3%,RSD=1.45%(n=6)。结论:该法快速、准确、可靠;可作为无敌丹胶囊的质量控制。

Objective： A specific HPLC method was developed for the measurement of Loureirin A in Wudidan Capsules.Methods： The sample was analyzed on Agilent ZORBAX XDB C18（250mm×4.6mm,5μm）column with mobile phase consist of acetic acid（1→90）-acetonitrile（63∶37）at flow rate of 1.0ml.min-1 and the detection wavelength was 260nm.Results：The calibration curves of Loureirin A was in good linearity over the ranges of 6.58ug·mL-1 ～65.8ug·mL-1,r was 0.99999,and the average recoveries（n=6）were 99.3% and RSD was 1.45%.Conclusion：The method is simple,rapid and accurate and can be used for quality control of Wudidan Capsules.