猪牙皂多糖提取工艺及体外抗氧化活性的研究

Study on the Extraction and Anti-oxidant Activity of Polysaccharide from Fructus Gleditsiae abnormalis Pericarp

目的:研究猪牙皂多糖的提取工艺条件及其抗氧化活性。方法:在对时间、温度、料液比进行单因素试验的基础上,采用正交试验优化猪牙皂多糖的提取工艺条件。抗氧化活性的研究采用邻苯三酚自氧化法。结果:料液比(A)、提取温度(B)、提取时间(C)对猪牙皂多糖提取影响的顺序为A>B>C。适宜的提取工艺条件为料液比1∶25、温度90℃、时间60min。0.9μg/mL的猪牙皂多糖溶液可消除50%的超氧自由基(即CC50=0.9μg/mL),对羟自由基(.OH)清除的CC50=1.74mg/mL。结论:猪牙皂多糖具有较强的清除超氧自由基和羟自由基的作用。

Objective：Study on the extraction technology and anti-oxidant activity of Pericarp Polysaccharide from Fructus Gleditsiae abnormalis.Method：Single factor and orthogonal tests were applied to optimize the extraction conditions of polysaccharide from Fructus Gleditsiae abnormalis pericarp,including the ratio of raw materials to solvent,temperature,time.Anti-oxidation was detected with pyrogallol autoxidation and Fenton reaction.Result：The optimum extraction conditions were the ratio of raw materials to solvant 1∶25（w/v）,90℃ for 60 minutes.Anti-oxidation tests show that the polysaccharides concentration required to clear 50% O2-·is 0.9μg/ml and the concentration required to clear 50% ·OH is 1.74mg/ml.Conclusion：Pericarp polysaccharides from Gleditsiae abnormalis appear strong antioxidation.