中国被毛孢发酵物对NOD小鼠1型糖尿病的预防作用

Prevention of strain fermentation in Hirsutella sinensis on type 1 diabetes of non-obese diabetic mice

目的观察中国被毛孢发酵物对自发性1型糖尿病NOD小鼠的预防作用。方法利用NOD小鼠1型自发性糖尿病模型,连续10周ig给予1 g/kg中国被毛孢发酵物后,观察NOD小鼠的体质量、血糖、发病率、谷氨酸脱羧酶（GAD）抗体水平、胰岛病变和胰腺纤维化的变化。结果 NOD小鼠给予1 g/kg中国被毛孢发酵物后,第6~10周时血糖和发病率明显降低（P〈0.05、0.01）,小鼠血清中GAD抗体明显降低（P〈0.01）,小鼠胰岛病变和胰腺纤维化评分值显著降低（P〈0.05、0.01）,胰腺结构较为清晰,胰岛有少量炎症细胞浸润,胰岛β细胞未见异常。

Objective To observe the prevention of strain fermentation in Hirsutella sinensis on spontaneous type 1 diabetes of non-obese diabetic（NOD） mice.Methods The NOD mice with spontaneous type 1 diabetes were administrated by 1 000 mg/kg strain fermentation in H.sinensis for 10 weeks.The index including body weight,blood glucose,incidence,GAD antibody,the islet pathological change,and pancreas fibrillation were tested.Results The levels of blood glucose and incidence decreased significantly after treatment by strain fermentation in H.sinensis（1 g/kg） for 6—10 weeks（P0.05 and 0.01）.GAD Antibody was depressed obviously（P0.01） and the islet pathological change and pancreas fibrillation grade decreased remarkably（P0.05 and 0.01）.The construction of pancreas was vivid,the inflamma-tory cell infiltration accident in islet and the β cell showed no abnormality.Conclusion The strain fermentation in H.sinensis has obvious prevention in spontaneous type 1 diabetes of NOD mice.