纤维素酶的固体发酵研究

Studies on Solid State Fermentation of Cellulase

对五种纤维素酶产生菌进行了酶系分析。在稻草料：麸皮=７：３，加水比１：３，硫酸铵添加量３％，初始ｐＨ６．０，培养温度２８－３０℃的优化条件下，培养康氏木霉（Ｔｒｉｃｈｏｄｅｒｍａ Ｋｏｎｉｇｉｉ）Ｈ５，发酵８４小时，可使ＣＭＣ酶活力达到１８６０ｕ／ｇ干曲。

Enzyme systems of five strains producing cellulase are analyzed.Trichoderma Konigii H5 isselected as test strain. Under optimal culture conditions, rice straw powers wheat bran is 7:3; the additionrate of wator is 1:3;the addition of (NH4)2SO4 is 3%; the initial pH is 6.0: the culture temperature is 28℃-30℃: the fermentation time is 84h. The CMC enzymatic activity achieved 1860u/g dry leaven.