斜卧青霉纤维素酶和木聚糖酶高产菌株的选育

Screening of Cellulase and Xylanase High-Yielding Penicillium decumbens Strain

以纤维素酶高产菌株斜卧青霉A50为出发菌株,通过紫外诱变原生质体获得1株木聚糖酶活力提高80%而纤维素酶活力没有改变的6号菌。蛋白质电泳和酶谱检测结果显示,纤维素酶谱基本无差别,而木聚糖酶谱显示6号菌比A50多了一条带。6号菌优化后的产酶培养基组成为：麸皮7%、葡萄糖0.1%,该条件下,纤维素酶活为19.7IU/mL,木聚糖酶活力为215.4IU/mL。

Cellulase high-yielding Penicillium decumbens A50 was used as a starting strain in this study and obtained mutant strain No.6 with xylanase increased by 80% without changing A50’s cellulase activity through UV treatment of its protoplasts. The results of protein electrophoresis and SDS-PAGE and zymogram revealed that mutant strain No.6 produced basically undifferentiated cellulase, but zymogram of new xylanase had one more band as compared with A50. The optimum medium for strain No.6 strain was composed of bran 7% and glucose 0.1%. Under this condition, the activity of the xylanase and cellulase was 215.4 IU/mL and 19.7 IU/ml respectively.