去卵巢大鼠脑内凋亡基因蛋白表达及半枝莲黄酮的干预作用

Effect of flavonoids from Scutellaria barbata on protein expression of apoptotic genes in the brain of ovariectomized rats

目的:探讨去卵巢大鼠脑内凋亡基因Bcl-2,Bax和p53的蛋白表达及半枝莲黄酮(Scutellaria barbata flavonoid,SBF)的干预作用。方法:摘除3月龄雌性Sprague-dawley(SD)大鼠卵巢10个月,模拟老年性痴呆(Alzheimer's disease,AD)模型,采用免疫组化法检测脑内凋亡基因Bcl-2,Bax和p53的蛋白表达,评价SBF对摘除卵巢诱导大鼠神经细胞凋亡的抑制作用。结果:摘除大鼠双侧卵巢可使大脑皮质中Bcl-2,Bax和p53的表达异常改变,Bcl-2阳性表达神经元数量明显减少,Bax和p53阳性表达神经元数量明显增多。去卵巢大鼠连续36 d灌胃(ig)SBF17.5,35和70 mg.kg-1可不同程度地改善去卵巢大鼠大脑皮层凋亡基因的异常表达。结论:摘除大鼠卵巢10个月可以诱导神经细胞凋亡,SBF对神经细胞凋亡有对抗作用,提示SBF可能有利于对AD的治疗。

Objective： To study the protein expression of apoptotic genes in the brain of ovariectomized rats and the effect of flavonoids from Scutellaria barbata（SBF）.Methods： Sprague-Dawley（SD） female rats（3 months old） were ovariectomized for 10 months to mimic AD-like disorder.The effect of SBF on neuron apoptosis in ovarietomized rats were evaluated by detecting protein expression of Bcl-2,Bax and p53 with immunohistochemical methods.Results： Ovarietomized rats exhibited abnormal changes in the protein expression of Bcl-2,Bax and p53 genes.Bcl-2 positive neurons were significantly increased,and Bax and p53 positive neurons were markedly decreased in ovarietomized rats.These abnormal changes in ovarietomized rats were attenuated by daily administrated SBF 17.5,35 and 70 mg·kg^-1 for consecutive 36 days.Conclusion： The ovariectomy can result in neuron apoptosis after 10 months in rats,and this apoptosis can be inhibited by SBF treatment for 36 days,suggesting that SBF may be beneficial to AD.