摘要
目的:了解慢性肝炎、肝硬化患者明胶酶A基因表达的变化。方法:慢性肝炎12例,肝硬化13例及正常对照8例肝穿取肝组织,用逆转录定量PCR方法,测定慢性肝病患者明胶酶A(MMP_2)的基因表达水平。结果:正常人有明胶酶A(MMP_2)的基因表达;慢性肝炎、肝硬化患者MMP_2基因表达较正常对照明显增强;慢性肝炎患者MMP_2基因表达水平明显高于肝硬化患者。结论:明胶酶A在慢性肝病的发生、发展中可能起重要作用。
Purpose: To investigate the alteration of gelatinase A (MMP2) gene expression in patients with chronic hepatitis and liver cirrhosis. Methods: 12 patients with chronic hepatitis, 13 patients with liv-er cirrhosis and 8 normal control were selected and liver tissue was obtained by liver biopsy. MMP2 gene ex-pression level was measured by quantitative analysis of reverse transcription polymerase chain reaction. Re-sults; MMP2 gene expression could be detected in normal subject. MMP2 gene expression levels in patients with chronic hepatitis and liver cirrhosis were higher than that of normal control. The gene expression level in patients with chronic hepatitis was higher in comparison with that in patients with liver cirrhosis. Con-clusion: MMP2 might play an important role in the pathogenesis and development of chronic liver disease.
出处
《临床消化病杂志》
1999年第2期65-67,共3页
Chinese Journal of Clinical Gastroenterology
关键词
肝炎
肝硬化
明胶酶A
基因表达
RT-PCR
Chronic hepatitis Liver cirrhosis Gelatinase A Gene expression Reverse transcrip-tion polymerase chain reaction
