实验性增殖性玻璃体视网膜病变动物模型的建立

ESTABLISHMENT OF EXPERIMENTAL PROLIFERATIVE VITREORETINOPATHY ANIMAL MODEL

目的比较3种不同方法建立增殖性玻璃体视网膜病变(proliferative vitreoretinopathy,PVR)动物模型,从中选取一种比较好的制作方法。方法新西兰白兔随机分为3组,玻璃体腔行气体压迫术后分别注入成纤维细胞、视网膜色素上皮(retinal pigment epithelial,RPE)细胞和富含血小板的血浆(platelet-rich plasma,PRP),观察各组注射后1、3、7、14、21、28、35d PVR的发生情况。结果成纤维细胞组与RPE细胞组相比,视网膜脱离的发生率差异无统计学意义(P>0.05);PRP组与成纤维细胞组和RPE细胞组相比,14d后视网膜脱离的发生率差异有统计学意义(P<0.05)。结论玻璃体腔注入成纤维细胞是一种较好的建立PVR模型的方法。

Objective Three kinds of rabbit models of proliferative vitreoretinopathy （PVR） were studied to seek a suitable PVR model. Methods Thirty New Zealand white rabbits were randomly divided into three groups and 10 rabbits for each, fibroblast cells, retinal pigment epithelial （RPE） cells and autologous platelet - rich plasma were injected into the midvitreous of each group respectively to establish PVR model. On the 1st, 3rd, 7th, 14th, 21st, 28th, and 35th day following injection , PVR was evaluated according to Ryan＇s grading criteria . Results On the 14 th , 21 st, 28 th ,and 35 th d after implantation, there was no significant statistical difference in the morbidity of retinal detachment between the fibroblast cell group and RPE cell group, significant differences were found between PRP group and fibroblast cell group, PRP group and RPE cell group after 14 th day. Conclusion PVR induced by implantation of fibroblast cells is more suitable as PVR model.